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Maddocks OD, Vousden KH (2011) Metabolic regulation by p53. Applying single-subjectexperimental research to inform clinical practice. Small, physiologicalamounts of folate are absorbed by specific carrier-mediated active transport in the intestinal mucosa.Large pharmacological doses may gain entry bypassive diffusion, but only a fraction is absorbed.Folic acid is rapidly extracted by tissues andstored in cells as polyglutamates. These discoveries should send oneback to the laboratory, not motivate an attempt to conceal the weakness of the study in vagueand ambiguous statements. Early evidence that transcrip-tion dysregulation may be one mechanism of mutant p53 included the nding thatActinomycin D, a potent inhibitor of transcription, blocked the reduction in apoptosisrate mediated by mutant p53 in response to treatment with doxorubicin or cisplatin [7].One of the rst studies to address the role of gene transcription in the mechanism ofaction of p53 GOF mutants was reported by Deb and colleagues in 2005 [21].

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